Genotyping is the process of finding differences in the genetic makeup of individual sheep in their DNA sequences.
DNA is mapped by finding the combination of single nucleotides that make up the DNA or the genome. The genome is the complete map of the individual’s DNA.
These single nucleotide polymorphisms (SNPs) show the differences between your sheep across the genome. At each SNP there are two alleles, one from each parent that indicate differences between sheep.
When you send your blood card or tissue sampling unit to a genotyping company (see bottom of page), they extract the DNA and map these SNPs. This shows what your sheep’s DNA looks like at each of these SNP locations.
The names of these SNPs relate roughly to the number of SNPs each chip has. For example, a 50K chip has roughly 50,000 SNP locations that will be identified.
If you subscribe and submit data to Sheep Genetics, your genotyped animals can receive genomically enhanced Australian Sheep Breeding Values (ASBVs). These genotypes estimate how related your animals are to the rest of the animals in the evaluation.
ASBVs estimate what genes an animal will on average give to their offspring. Breeding values are estimated based on the traits recorded for an animal and its relatives. Therefore, if your animals are related to genotyped animals in the database, these related animals will contribute information to your animals breeding values. If there is enough information to estimate an accurate breeding value, it will be reported as an ASBV.
These relationships are important for traits that are hard or expensive to measure such as eating quality, traits measured in adults and disease traits.
Genotyping will help provide information but the best way to get a breeding value is by measuring your own sheep. For more information about how to get involved, contact Sheep Genetics.
These breeds have enough animals in the reference population to use genotypes in the single step analysis
|Merino||White Suffolk||Poll Dorset|
|Border Leicester||Composite Maternal||Coopworth|
Because we have more data from some breeds than others in the database the benefit of genotyping varies between breeds.
MLA is working with the industry to include more breeds into the reference flock. Contact Sheep Genetics to discuss the suitability of your breed.
MLA manage and maintain Australia’s sheep industry genomic database.
This database includes:
MLA own the intellectual property of genotypes from the Sheep CRC and have a license to use new genotypes for parentage verification and analysis.
MLA hold all genotypes in one industry database so:
This means whichever company you use, all genotypes can be used together for parentage and genetic evaluation.
A flock profile test genotypes 20 animals to get an idea of how a flock benchmarks to industry, expressed as ASBVs.
Commercial Merino breeders can use a flock profile test to predicts a flock’s genetics compared to Sheep Genetics data.
Talk to one of the genomics companies that accept genotypes. It’s important to genotype 20 random animals from your flock, from the same year drop.
The flock profile gives average breeding values of a flock for important traits. These numbers are the average estimate for your flock and reflect the genetic merit of your ram team used five to seven years ago.
A flock profile also shows where a flock sits compared to other Merino flocks in the analysis.
There are a few genetic conditions or traits controlled by one or a small number of genes. These genes are found using single nucleotide polymorphisms (SNPs).
A marker report include tests for the following:
Breeders are advised to seek advice when using genetic conditions and recessive genes in a breeding program. Not all genetics conditions and recessive genes have been validated as functional for all breeds.
Parentage assignment uses single nucleotide polymorphism (SNP) information to allocate potential parents.
Potential parents are checked to find who shares at least one allele at each loci. For example, potential parents are ruled out in the purple box because they do not share enough alleles as the animal tested.
To assign parents, all potential sires and all dams need to be genotyped. This assignment to parents rules out potential sires and dams that do not match the progeny. For this reason, it is important to submit accurate and complete sire and dam lists for the best results.
The parentage assignment can only use the lowest single nucleotide polymorphism (SNP) coverage available in the potential candidates.
If all offspring are genotyped with 1,200 SNPs but some potential parents are genotyped with old 170 SNP panels, then only 170 SNPs are used for the parentage test.
The table below displays the genotyping providers and the products they offer.
|Parentage only||Parentage and genomic test||Flock profile||Marker tests|
P: +64 800 362 522
P: 0459 855 862
P: 07 37362134
Weatherbys Scientific Australia
P: 0424 004 622
P: 0417 938 067